Intrinsic Conformational Plasticity of Native EmrE Provides a Pathway for Multidrug Resistance
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منابع مشابه
Intrinsic Conformational Plasticity of Native EmrE Provides a Pathway for Multidrug Resistance
EmrE is a multidrug resistance efflux pump with specificity to a wide range of antibiotics and antiseptics. To obtain atomic-scale insight into the attributes of the native state that encodes the broad specificity, we used a hybrid of solution and solid-state NMR methods in lipid bilayers and bicelles. Our results indicate that the native EmrE dimer oscillates between inward and outward facing ...
متن کاملProtonation-dependent conformational dynamics of the multidrug transporter EmrE.
The small multidrug transporter from Escherichia coli, EmrE, couples the energetically uphill extrusion of hydrophobic cations out of the cell to the transport of two protons down their electrochemical gradient. Although principal mechanistic elements of proton/substrate antiport have been described, the structural record is limited to the conformation of the substrate-bound state, which has be...
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Multidrug resistance (MDR) is a major public health problem that reduces the efficacy of antibiotics in the treatment of infections. One of the most commonmechanisms in bacteria for conferring MDR is the coupling of drug efflux with the proton motive force. A prototype for studying ion-coupled active transport is the polytopic E. coli membrane protein EmrE, a member of the small multidrug resis...
متن کاملStructure, dynamics, and substrate-induced conformational changes of the multidrug transporter EmrE in liposomes.
EmrE, a member of the small multidrug transporters superfamily, extrudes positively charged hydrophobic compounds out of Escherichia coli cytoplasm in exchange for inward movement of protons down their electrochemical gradient. Although its transport mechanism has been thoroughly characterized, the structural basis of energy coupling and the conformational cycle mediating transport have yet to ...
متن کاملConformational changes in the multidrug transporter EmrE associated with substrate binding.
EmrE is a bacterial multidrug transporter of the small multidrug resistance family, which extrudes large hydrophobic cations such as tetraphenylphosphonium (TPP(+)) out of the cell by a proton antiport mechanism. Binding measurements were performed on purified EmrE solubilized in dodecylmaltoside to determine the stoichiometry of TPP(+) binding; the data showed that one TPP(+) molecule bound pe...
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ژورنال
عنوان ژورنال: Journal of the American Chemical Society
سال: 2014
ISSN: 0002-7863,1520-5126
DOI: 10.1021/ja503145x